Objective:To understand the influence of personal hygiene behaviors on reproductive tract infections (RTIs) and to provide evidence for the intervention among married women at childbearing age Methods:A total of 1 631 married women in 60 residence neighborhoods from 4 streets and 2 stagnant enterprises were selected by multistage cluster random sampling and interviewed face-to-face through questionnaires, laboratory tests assay and gynecological examination. All data were analyzed by SAS 8.2. Results:The positive detection rates of five target pathogens, trichomonas vaginalis, candida albicans, G.vaginalis, chlamydia trachomatis and Neisseria gonorrhoeae, in the study were 3.04%, 5.04%, 1.52%, 6.68%, and 0.12%, respectively. Personal hygiene practices and sexual behaviors among married women in the study were good in general. The vast majority of women (98.77%) rinsed their vulvae every day and more than half of the women (53.03%) took bath every day. In terms of the prevalence of endogenous infection, there were statistical differences between women who had different frequencies of rinsing their vulvae and taking bath; and for the prevalence of sexually transmitted infection, there were significant differences between women whose husband had different frequency of washing their external genital organs before sexual intercourses each time. The mostly adopted contraceptive methods among women ranked by frequency were intra-uterine devices (IUD) (68.69%), male condom (16.69%) and safe period (6.20%), among which male condom could prevent sexually transmitted infections. Multiple logistic regression analyses indicated that the frequency of taking bath, the husband washing his external genital organ before sexual intercourse and the age of marriage were the influencing factors of RTIs, too. Conclusion:It is very urgent that health educational intervention of RTIs among married women be conducted and especially the knowledge on condom’s double effects on contraception and prevention of RTIs be disseminated to urge women to practice hygiene behavior to achieve the aim of preventing RTIs. Objective: To understand the influence of personal hygiene behaviors on reproductive tract infections (RTIs) and to provide evidence for the intervention among married women at childbearing age Methods: A total of 1 631 married women in 60 residence neighborhoods from 4 streets and 2 stagnant enterprises were selected by multistage cluster random sampling and interviewed face-to-face through questionnaires, laboratory tests assay and gynecological examination. All data were analyzed by SAS 8.2. Results: The positive detection rates of five target pathogens, trichomonas vaginalis, candida albicans, G .vaginalis, chlamydia trachomatis and Neisseria gonorrhoeae, in the study were 3.04%, 5.04%, 1.52%, 6.68%, and 0.12% respectively, respectively. Personal hygiene practices and sexual behaviors among married women in the study were good in general. The vast majority of women (98.77%) rinsed their vulvae every day and more than half of the women (53.03%) took bath every day. In terms of the prevalence of endo genous infection, there were statistical differences between women who had different frequencies of rinsing their vulvae and taking bath; and for the prevalence of sexually transmitted infection, there were significant differences between women the past husband had different frequency of washing their external genital organs before sexual intercourses each time. The mostly adopted contraceptive methods among women ranked by frequency were intrauterine devices (IUD) (68.69%), male condom (16.69%) and safe period (6.20%), among which male condom could prevent sexually transmitted transmitted. Multiple logistic regression analyzes indicated that the frequency of taking bath, the husband of washing his external genital organ before sexual intercourse and the age of marriage were the influencing factors of RTIs, too. Conclusion: It is very urgent that health educational intervention of RTIs women be conducted and especially the knowledge on condom’s double effects on contraceptionand prevention of RTIs be disseminated to urge women to practice hygiene behavior to achieve the aim of oplestive RTIs.