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目的:探讨不同电场强度的不可逆电穿孔(IRE)对于肿瘤的消融效果以及其所引起的肿瘤免疫反应。方法:将B16黑色素瘤细胞接种于C57小鼠,制作黑色素瘤小鼠模型,应用不可逆电穿孔仪对肿瘤进行消融,通过测量肿瘤生长大小研究不同场强下的消融效果。通过CD4、CD8a免疫组化染色初步了解不同场强激发的免疫反应的强弱,从而选择最佳消融参数。结果:随着电场强度增加,肿瘤消融效果逐渐趋于理想,然而平板电极夹之间过高电场引发的热效应则不利于后续抗肿瘤免疫反应的激活。选择合适的电场,通过增加电击组数可以达到良好的治疗效果。结论:IRE对肿瘤的消融优势在于它能够很好的激活机体的抗肿瘤免疫反应,因此,选择合适的电场增加电击组数可以起到很好的抗肿瘤作用,具有很好的应用前景。
Objective: To investigate the ablation effect of irreversible electroporation (IRE) with different electric field intensity and the tumor immune response caused by it. Methods: B16 melanoma cells were inoculated into C57 mice to make a mouse model of melanoma. The tumors were ablated by irreversible electroporation. The ablation effect under different field strengths was studied by measuring the size of tumor growth. By CD4, CD8a immunohistochemical staining preliminary understanding of different field strength of the immune response to stimulate the strength, so as to select the best ablation parameters. Results: With the increase of electric field intensity, the tumor ablation effect gradually became ideal. However, the thermal effect caused by excessive electric field between plate electrodes was not conducive to the subsequent activation of anti-tumor immune response. Select the appropriate electric field, by increasing the number of shock groups can achieve good therapeutic effect. Conclusion: The advantage of IRE in tumor ablation is that it can well activate the anti-tumor immune response of the body. Therefore, choosing an appropriate electric field to increase the number of shock groups can play a good anti-tumor effect and has good application prospect.