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为研究鼻中隔不同部位生后发育情况,作者们对18例施行鼻中隔保守性成形术者(男8女10,年龄在6～10岁者5例、11～15岁者7例,20～40岁者5例)(原文共17例——摘者),于手术时在鼻中隔的五个不同部位:即软骨前游离缘、上颌骨前上区、后区(中隔软骨与筛骨结合处之前)、中央区及中隔软骨尾部,各取一小条软骨,剥离软骨膜后,浸泡在两种溶液中。一种为标有硫的硫酸纳,用以研究蛋白多糖的合成;一种标有氢胸腺嘧啶核苷,用以研究DNA的合成。在37℃温度下孵育48小时;取出标本用清水冲洗2小时;干燥后放在浓缩的甲酸中、在70℃温度
In order to study the postnatal development of different parts of the nasal septum, the authors evaluated 18 cases of conservative surgery for nasal septoplasty (8 males and 10 females, 5 males and females aged 6 to 10, 7 and 11 to 15 years old, 20 to 40 years old In 5 cases) (17 cases of the original - excerpt), at surgery in five different parts of the nasal septum: the anterior cartilage, the anterior superior maxilla, the posterior area (septal cartilage and ethmoid junction before ), The central area and septal cartilage tail, each take a small piece of cartilage, stripping perichondrium, soaked in two solutions. One is sulfur-labeled sodium sulphate to study the synthesis of proteoglycans; one is labeled with hydrogen thymidine to study the synthesis of DNA. Incubated for 48 hours at a temperature of 37 ° C; the specimens were washed with water for 2 hours; dried and placed in concentrated formic acid at a temperature of 70 ° C