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目的探讨黄芪对糖尿病大鼠肾组织中血管生成素(Ang)及其受体Tie-2和血管内皮生长因子(VEGF)表达的影响。方法18只SD大鼠随机分为正常对照组、糖尿病组和糖尿病黄芪治疗组。实时PCR和免疫组化法分别检测3组大鼠肾组织中Ang-1、Ang-2、Tie-2和VEGF的mRNA和蛋白表达。结果与正常对照组比较,糖尿病组大鼠肾组织VEGF mRNA以及VEGF、Ang-2和Tie-2蛋白表达明显升高(P<0.01);与糖尿病组大鼠比较,糖尿病黄芪治疗组大鼠肾组织VEGF mRNA表达显著升高,而Tie-2蛋白表达显著下调(P<0.01),两组间Ang-1和Ang-2的mRNA和蛋白表达未见统计学差异(P>0.05)。结论黄芪对糖尿病大鼠肾组织Ang表达的调节作用不明显,但可下调其受体Tie-2的表达。
Objective To investigate the effect of astragalus on the expression of angiopoietin (Ang), its receptors Tie-2 and vascular endothelial growth factor (VEGF) in the kidney of diabetic rats. Methods Eighteen SD rats were randomly divided into normal control group, diabetic group and diabetic jaundice treatment group. Real-time PCR and immunohistochemistry were used to detect mRNA and protein expression of Ang-1, Ang-2, Tie-2, and VEGF in renal tissues of the three groups. Results Compared with normal control group, the expression of VEGF mRNA and VEGF, Ang-2 and Tie-2 protein in kidney tissue of diabetic group was significantly increased (P<0.01); The expression of VEGF mRNA was significantly increased in tissue, while the expression of Tie-2 protein was significantly down-regulated (P<0.01). There was no statistical difference in the expression of Ang-1 and Ang-2 mRNA and protein between the two groups (P>0.05). Conclusion The effect of Huangqi on the expression of Ang in renal tissues of diabetic rats is not obvious, but the expression of its receptor Tie-2 can be down-regulated.