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白细胞介素-2(Interlenkin-2,IL-2)是具有广泛免疫调节作用的淋巴因子,但用常规方法提取非常困难。为深入研究小鼠IL-2的体内体外功能,我们利用基因工程技术,成功地在大肠杆菌中表达出有生物活性的小鼠IL-2。 我们从小鼠IL-2cDNA克隆pcD-IL-2中通过限制性内切酶消化获得IL-2cDNA插入片段,将其连接到带有λ噬菌体PL启动子的表达质粒载体pMS_
Interleukin-2 (IL-2) is a lymphokine that has a wide range of immunomodulatory effects, but extraction using conventional methods is very difficult. To further investigate the in vivo and in vitro functions of mouse IL-2, we successfully expressed the bioactive mouse IL-2 in E. coli using genetic engineering techniques. We obtained the IL-2 cDNA insert by restriction endonuclease digestion from mouse IL-2 cDNA clone pcD-IL-2 and ligated into the expression plasmid vector pMS_l with lambda phage PL promoter