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目的:研究双歧杆菌分泌型黏附素对大鼠缺血-再灌注(I/R)后肠黏膜屏障的防护作用。方法:将54只大鼠随机分为假手术组(对照组)、模型组(I/R组)和黏附素预处理组(实验组),每组各24只。于造模成功后6 h、第1、第4和第7天,分别取6只大鼠的血和小肠标本,观察小肠组织病理改变,检测各时间点血中肿瘤坏死因子-α(TNF-α)I、L-6I、L-10、二胺氧化酶(DAO)和D-乳酸(D-LAC)的活性和含量。结果:与对照组比较I,/R组血中TNF-αI、L-6I、L-10、DAO和D-乳酸水平在各时相点均升高(P<0.05);与I/R组比较,实验组各时间点IL-6和DAO水平明显降低(P<0.05),TNF-α浓度术后第1天低于I/R组(P<0.05),术后第4和第7天实验组大鼠血浆D-LAC浓度明显低于I/R组(P<0.05),小肠病理改变较I/R组减轻(P<0.05)。结论:双歧杆菌黏附素对大鼠I/R后肠黏膜屏障具有防护作用,能减轻肠黏膜的I/R损伤。
Objective: To study the protective effect of Bifidobacterium sepsis on intestinal mucosal barrier after ischemia / reperfusion (I / R) in rats. Methods: 54 rats were randomly divided into sham operation group (control group), model group (I / R group) and adhesin pretreatment group (experimental group), 24 rats in each group. At 6 h, 1, 4 and 7 days after successful model establishment, blood and small intestine specimens of 6 rats were respectively taken for observing pathological changes of small intestine. Tumor necrosis factor-α (TNF- α) I, L-6I, L-10, diamine oxidase (DAO) and D-lactic acid (D-LAC) Results: Compared with the control group, the levels of TNF-αI, L-6I, L-10, DAO and D-lactic acid in I and / R groups increased at each time point The levels of IL-6 and DAO in the experimental group were significantly lower than those in the I / R group (P <0.05) on the first day after operation, and the levels of IL-6 and DAO in the experimental group were significantly decreased The concentration of plasma D-LAC in the experimental group was significantly lower than that in the I / R group (P <0.05), and the pathological changes in the small intestine were relieved compared with the I / R group (P <0.05). Conclusion: Bifidobacterium adrexin plays a protective role in the intestinal mucosal barrier of I / R rats, and can reduce I / R injury of intestinal mucosa.