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定居因子CFA/I和CS6是肠毒素大肠杆菌 (ETEC)中重要的两种优势抗原 ,是ETEC疫苗研制的首选组分。采用基因重组技术将二者构建在以asd基因为选择标记的重组质粒上 ,与asd基因缺失突变型减毒福氏志贺氏菌FWL0 1构成宿主 载体平衡致死系统。实验结果表明 ,重组疫苗候选株能够稳定表达CFA/I和CS6抗原 ,并可在菌体表面形成相应菌毛。重组菌口服免疫BALB/c小鼠后 ,可诱生相应的抗CFA/I和CS6的特异性血清抗体IgG和分泌型抗体sIgA ,说明以志贺氏菌为载体 ,可以构建同时表达多个定居因子抗原的ETEC多价菌苗
Settling factors CFA / I and CS6 are two important antigens in enterotoxigenic Escherichia coli (ETEC), and are the first choice of ETEC vaccine. The recombinant plasmids were constructed based on the asd gene as a selectable marker by using gene recombination technology, and the lethally-modified lethal system of host vector was constructed with the attenuated aspergillus oryzae FWL0 1 asd gene deletion mutant. The experimental results show that recombinant vaccine candidate can stably express CFA / I and CS6 antigens, and can form corresponding pili on the cell surface. After oral immunization of recombinant BALB / c mice, the corresponding anti-CFA / I and CS6-specific serum IgG and secreted antibody sIgA were induced, indicating that Shigella can be used as a vector to construct multiple simultaneous colonies Factor ETEC multivalent vaccine