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采用 Hoechst332 5 8荧光染色和透射电镜观察了受流感病毒感染的狗胚肾传代细胞的形态学特征 .结果表明 ,病毒感染 2 4h后 ,MDCK细胞产生明显的染色质凝聚 ,核碎裂及形成凋亡小体等现象 .早期的形态改变是染色质浓集成块 ,分散排列在核膜周边 ,胞质亚显微结构正常 ;随后出现胞浆膜或核膜内陷 ,将细胞分割成多个大小不等的不连续凋亡小体 ,小体外有质膜包裹 ,多数小体内有核质成份 .流感病毒感染可诱导 MDCK细胞凋亡
Hoechst3328 fluorescence staining and transmission electron microscopy were used to observe the morphological characteristics of the passaged germ cells infected by influenza virus.The results showed that chromatin condensation and nuclear fragmentation were observed in the MDCK cells after 24 h of virus infection Dead bodies and other phenomena.Early morphological changes are concentrated chromatin into lumps, scattered around the nuclear membrane, cytoplasmic sub-microstructure is normal; followed by cytoplasmic membrane or nuclear membrane retraction, the cell is divided into multiple sizes Ranging from discontinuous apoptotic bodies, small bodies with plasma membrane parcels, the majority of small bodies have nuclear components. Influenza virus infection can induce apoptosis of MDCK cells