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目的 了解我国大兴安岭地区可疑人群是否存在人埃立克体感染。方法 用 16SrRNA基因特异引物进行半巢式多聚酶链反应及基因序列分析技术 ,检测大兴安岭地区的一些人血标本中查菲埃立克体 (Ehrlichiachaffeensis,EC)和人粒细胞埃立克体 (humangranulocyticehrlichia)的16SrRNA基因 ,并进行序列分析 ,将测出的序列与GenBank中注册的基因序列进行最大同源性比较。结果 从蜱咬后出现发热等病症的 35例病人和近一年内有蜱咬史的林场工人血中检测到这两种埃立克体的 16SrRNA基因片段 ;对 1份可疑埃立克体病人血标本进行了人粒细胞埃立克体 16SrRNA全基因扩增 ,测出的序列与美国 1株人粒细胞埃立克体的该基因仅差 1个碱基。结论 初步认为我国大兴安岭地区存在人埃立克体感染人群
Objective To understand the existence of Ehrlichosis in suspicious people in Daxinganling area in China. Methods Echlichiachaffeensis (EC) and humangranulocyticehrlichia were detected by semi-nested polymerase chain reaction (PCR) and sequence analysis in 16S rRNA gene-specific primers. Of the 16S rRNA gene, and the sequence analysis, the measured sequence and GenBank registered gene sequence for maximum homology comparison. Results The 16S rRNA gene fragments of Ehrlichiae were detected in 35 patients with fever and other fever after tick bite and in farm workers with tick bites in the past year. The whole genome of human granulocyte Ehrlichiae 16SrRNA was amplified. The sequence was only 1 base different from that of Ehrlichia granulosus in USA. Conclusion It is preliminarily thought that Ehrlichiae infection exists in Daxinganling area of our country