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目的:分析何首乌中核苷类成分积累的动态变化,以确定何首乌的适宜采收期。方法:采用UPLCQTRAP-MS/MS技术测定不同采收期何首乌中9种核苷类成分的含量。结果:何首乌核苷类成分中,以尿苷、腺嘌呤、鸟苷、胞苷含量较高;不同采收期何首乌中核苷类成分含量呈峰谷状变化,12月份含量相对较高。结论:何首乌核苷类成分的积累与生长期密切相关,其积累高峰期与当地传统采收期基本一致。
OBJECTIVE: To analyze the dynamic changes of accumulation of nucleosides in Polygonum multiflorum Thunb. So as to determine the suitable harvest period of Polygonum multiflorum. Methods: UPLCQTRAP-MS / MS was used to determine the content of nine nucleosides in Polygonum multiflorum collected at different harvesting times. Results: The contents of uridine, adenine, guanosine and cytidine were higher in the nucleosides of Polygonum multiflorum. The content of nucleosides in the Polygonum multiflorum was changed from peak to valley in different harvest periods, and the content of nucleosides in December was relatively high. Conclusion: The accumulation of nucleosides of Polygonum multiflorum is closely related to the growth period, and the accumulation peak is consistent with the traditional harvesting period.