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目的 通过检测恢复期SARS患者外周血淋巴细胞亚群分布及其与T淋巴细胞核仁形成区嗜银蛋白 (Ag NOR)含量的相关性分析 ,探讨恢复期SARS患者的免疫状态及淋巴细胞亚群与活性状态改变的关系。方法 以流式细胞术检测患者外周血淋巴细胞亚群 ,用KL型免疫图像分析系统检测外周血T淋巴细胞Ag NOR ,对两者进行相关分析。结果 恢复期患者T淋巴细胞总数及CD4 +亚群基本正常 ,而CD8+亚群偏高 ,CD4 +/CD8+降低 ,CD4 +/CD8+<1的比例占 37.4 % ,激活T细胞中以CD8+细胞为主。B细胞比例正常。NK细胞明显低于对照组。重症患者CD4 +、CD4 +/CD8+、CD1 9+CD5+降低及CD8+、CD3+HLA DR+升高。 50岁以上及使用大剂量激素患者CD3+HLA DR+升高。淋巴细胞Ag NOR的含量 (IS % )在正常范围 ,但患者IS值呈偏态分布 ,低于正常范围者占 4 4.99%。淋巴细胞Ag NOR的含量与CD3+、NK、CD3+HLA DR+、CD3+CD2 5+在统计学上具有相关性。结论 恢复期SARS患者免疫功能趋于恢复正常 ,但部分患者的淋巴细胞亚群数量及淋巴细胞活性仍未恢复正常 ,这些病人在临床症状得到改善之后 ,尚需一定时间的观察随访 ,以了解SARS病毒对人体免疫机能的长期影响。
Objective To investigate the distribution of peripheral blood lymphocyte subsets and their relationship with the content of Ag-NOR in convalescent SARS patients and to investigate the relationship between immunocompetence and lymphocyte subsets in patients with convalescent SARS Changes in the relationship between the state of activity. Methods The peripheral blood lymphocyte subsets were detected by flow cytometry. The Ag NOR of peripheral blood T lymphocytes was detected by KL immunoassay system, and the correlation was analyzed. Results The total number of T lymphocytes and CD4 + subsets in convalescent patients were basically normal while the proportion of CD8 + subsets was higher than that in CD4 + / CD8 + cells. The proportion of CD4 + / CD8 + 1 was 37.4% . B-cell ratio is normal. NK cells were significantly lower than the control group. Severe patients with CD4 +, CD4 + / CD8 +, CD19 + CD5 + and CD8 +, CD3 + HLA DR + increased. Patients over 50 years of age and use of high-dose hormone CD3 + HLA DR + increased. The content of AgNOR in lymphocytes (IS%) was in the normal range, but the IS value of the patients showed a skewed distribution, accounting for 4. 4.99% below the normal range. The level of AgNOR in lymphocytes was statistically correlated with CD3 +, NK, CD3 + HLA DR +, CD3 + CD25 +. Conclusion The immune function of SARS patients in convalescent phase tends to return to normal, but the number of lymphocyte subpopulation and lymphocyte activity in some patients have not returned to normal. After these patients’ clinical symptoms have been improved, they still need some time to observe and follow up to understand SARS Long-term effects of viruses on human immune function.