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为了筛选光温敏雄性不育系(PTGMS)小麦穗部的EST-SSR分子标记,探讨其EST-SSR标记用于小麦品种遗传差异研究的可行性,用SSRSCAN软件对PTGMS小麦穗部的3 264条EST序列进行SSR位点查找,结果得到108条含有SSR标记的序列。设计64对引物并进行PCR扩增及非变性聚丙烯酰胺凝胶(PAGE)电泳。结果显示,64对引物中有41对能在42个小麦品种中扩出PCR条带,有多态性条带的为36对,具有较高的扩增效率。聚类分析结果显示,42个小麦品种的遗传相似系数在0.64~0.87之间,3个PTGMS小麦品种具有较高的遗传相似系数。
In order to screen the EST-SSR molecular markers of PTGMS wheat spikes and to explore the feasibility of EST-SSR markers for the genetic differences of wheat cultivars, the SSRSCAN software was used to analyze the genetic diversity of PTGMS wheat spikes SSR locus search of the EST sequence results in 108 sequences containing the SSR marker. Sixty-four pairs of primers were designed and subjected to PCR amplification and non-denaturing polyacrylamide gel (PAGE) electrophoresis. The results showed that 41 out of 64 pairs of primers amplified PCR bands in 42 wheat cultivars, and 36 pairs of polymorphic bands showed high amplification efficiency. Cluster analysis showed that the genetic similarity coefficients of 42 wheat cultivars ranged from 0.64 to 0.87. Three PTGMS wheat cultivars had higher genetic similarity coefficients.