用分离培养法和ＥＬＩＳＡ法对受嗜肺巴氏杆菌隐性感染的昆明小鼠群中不同鼠龄段小鼠进行的检测结果表明：带菌率随着鼠龄的增加而降低，而抗阳体阳性率则随鼠龄增加而升高。在方法学上，低鼠龄段小鼠，分离培养法明显优于ＥＬＩＳＡ法，而在老龄鼠则反之；用ＥＬＩＳＡ法进行该菌的常规监测时，选用老龄鼠将是保证监测质量的关键 The isolation and culture methods and ELISA method in mice infected by Pasteurella pneumophila latent infection in mice of different mouse age group mice test results showed that: the carrier rate decreased with the increase of the mouse’s age, and anti-Yang The positive rate increases with the increase of age. Methodologically, the low mouse-age mice, isolation and culture method was significantly better than the ELISA method, while in the case of aging mice; the routine monitoring of the bacteria by ELISA, the use of old mice will be the key to ensuring the quality of monitoring