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目的:探讨复方乌梅散对人胃癌SGC-7901细胞株裸鼠移植瘤生长及血管生成相关因子COX-2、VEGF-C表达的影响。方法:将人胃癌细胞SGC-7901通过皮下种植接种于裸鼠腋下,制备裸鼠移植瘤模型,造模成功后随机将裸鼠分为模型组、复方乌梅散小剂量组(10g/kg)、复方乌梅散中剂量组(15g/kg)、复方乌梅散大剂量组(20g/kg),每日灌胃1次,共11次,末次给药后剥离瘤组织称质量,计算移植瘤体积、抑瘤率。应用qRT-PCR检测瘤组织中COX-2和VEGF-C mRNA表达水平。用免疫组织化学法和Western blot法分别检测各组肿瘤组织中COX-2和VEGF-C蛋白的表达。结果:复方乌梅散小剂量组、中剂量组、大剂量组抑瘤率分别为31.83%、34.83%、55.26%,大剂量组抑制肿瘤生长的作用明显增强。免疫组化结果显示复方乌梅散各组移植瘤组织中COX-2和VEGF-C表达均有降低趋势,Western blot结果显示复方乌梅散各组移值瘤组织中COX-2和VEGF-C蛋白表达均呈降低趋势,qRT-PCR检测显示复方乌梅散各组移植瘤COX-2、VEGF-C mRNA的表达明显下调。结论:复方乌梅散可抑制SGC-7901细胞裸鼠移植瘤的生长,抑制COX-2、VEGF-C的表达,且呈剂量依赖性。
Objective: To investigate the effects of Fufang Wumei Powder on the growth and the expression of COX-2 and VEGF-C of human gastric cancer SGC-7901 cells in nude mice. Method: The human gastric cancer cells SGC-7901 were inoculated in nude mice by subcutaneous implantation armpit, nude mice model was prepared, after a successful modeling Mice were randomly divided into model group, low dose group of compound ebony Powder (10g / kg) (15g / kg), high-dose compound group (20g / kg) and gavage once a day for 11 times. The mass of stromal tumor was calculated after the last administration, and the volume of tumor was calculated , Inhibition rate. QRT-PCR was used to detect the expression of COX-2 and VEGF-C mRNA in tumor tissue. The expressions of COX-2 and VEGF-C in tumor tissues were detected by immunohistochemistry and Western blot respectively. Results: The inhibitory rates of Fufang Wumei Powder in small dose group, medium dose group and high dose group were 31.83%, 34.83%, 55.26% respectively. The effect of high dose group on inhibiting tumor growth was significantly enhanced. Immunohistochemistry results showed tumor in each group ebony Powder Compound COX-2 expression and VEGF-C were reduced tendency, Western blot results showed Powder Compound Mume shift values of each group of neoplastic tissue COX-2 expression and VEGF-C All showed a decreasing tendency. The qRT-PCR results showed that the expression of COX-2 and VEGF-C mRNA in the xunfulai xue xue xue xue xue xue xue xue xue xue xue xin xiao xian xue xin xiao xian xing xin xiao xian xing xiao xiao xiao xian xing xin xing xing xing xue xing xue xing xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xue xing Conclusion: Fufang Wumei San can inhibit the growth of SGC-7901 cells in nude mice and inhibit the expression of COX-2 and VEGF-C in a dose-dependent manner.