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目的观察养心透毒方对柯萨奇病毒(CVB3)感染小鼠造成的CVB3病毒性心肌炎(VMC)的保护作用。方法将HeLa细胞培养的CVB3制成悬液,测定半数组织细胞感染量(TCID50),小鼠腹腔接种CVB3培养液建立VMC模型。小鼠随机分为正常组,模型组,利巴韦林+辅酶Q10(CoQ10)组,养心透毒方低、高剂量组,灌胃给药4周。应用酶联免疫法检测各组小鼠IgG和IgM阈值,测定血清肌钙蛋白T(cTnT)、白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)水平,组织匀浆测定心肌IL-2和TNF-α含量,HE染色观察小鼠的心肌组织病理形态学改变。结果养心透毒方低、高剂量组IgM、IgG阳性的动物数均少于模型组。模型组心肌组织及血清IL-2、TNF-α含量与正常组比较均有显著性差异(P<0.05),心肌组织有明显病理改变。养心透毒方可使小鼠心肌组织坏死程度减轻,心肌炎症浸润减少,降低组织和血清的IL-2和TNF-α含量(P<0.05)。结论养心透毒方能减少小鼠CVB3病毒的感染率,降低组织中炎症因子的水平,对小鼠早期VMC具有防治作用。
Objective To observe the protective effect of Yangxinjingdu on CVB3 viral myocarditis (VMC) in mice infected with Coxsackie virus (CVB3). Methods The suspension of CVB3 was cultured in HeLa cells and the half tissue cell infection (TCID50) was determined. The mice were intraperitoneally inoculated with CVB3 medium to establish a VMC model. Mice were randomly divided into normal group, model group, ribavirin + coenzyme Q10 (CoQ10) group, and low-dose high-dose group of Yangxinrendu. The mice were given intragastric administration for 4 weeks. The IgG and IgM thresholds of each group were detected by enzyme-linked immunosorbent assay. Serum troponin T (cTnT), interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) levels were measured. The contents of IL-2 and TNF-α in myocardium were measured by HE staining, and the histopathological changes of myocardial tissue were observed by HE staining. Results The number of IgM and IgG positive animals in the low-dose and high-dose groups was lower than that in the model group. The myocardial tissue and serum levels of IL-2 and TNF-α in the model group were significantly different from those in the normal group (P<0.05). There were obvious pathological changes in the myocardial tissue. Yang Xin Shen Du Fang can reduce the degree of myocardial necrosis in mice, reduce the infiltration of myocardial inflammation, and reduce the content of IL-2 and TNF-α in tissues and serum (P<0.05). Conclusion Yangxinjingdu can reduce the infection rate of CVB3 virus in mice and reduce the level of inflammatory cytokines in the mice. It has preventive and therapeutic effects on early VMC in mice.