论文部分内容阅读
目的 :研究无细胞短棒状杆菌纳米级制剂 (NCPP)体内抗肿瘤作用及机理。方法 :小鼠腹腔注射NCPP与短棒状杆菌制剂 (CPP) 14d后测定脾指数。小鼠腹腔注射艾氏腹水癌细胞 ,于注射癌细胞前 1d及注射后次日起 ,连续 (间隔 1d)腹腔注射NCPP与CPP 5次 ,计算小鼠存活率。小鼠腹腔注射NCPP和CPP 10d后测定腹腔巨噬细胞 (Mφ)吞噬率、吞噬指数、过氧化氢 (H2 O2 )和一氧化氮 (NO)水平、脾脏自然杀伤 (NK)细胞杀伤率及T细胞增殖指数。结果 :NCPP组和CPP组与对照组相比 :均有明显的抑瘤作用 ;脾指数均大于 2 .0 ;腹腔Mφ吞噬率、吞噬指数、产生过氧化氢 (H2 O2 )和一氧化氮 (NO)水平均有显著性提高 (P <0 .0 1) ;脾脏NK细胞杀伤率均有明显提高 (P <0 .0 5 ) ;脾脏T细胞增殖指数均无明显差异。上述所有实验中 ,NCPP和CPP 2组间均无明显差异。结论 :NCPP具有与CPP相同的脾激活及抑瘤作用 ,其机理主要是通过激活Mφ及NK细胞吞噬、杀伤功能所致。
Objective: To study the anti-tumor effect and mechanism of NCPP in vivo. Methods: Spleen index was measured after intraperitoneal injection of NCPP and Corynebacterium parvum preparations (CPP) for 14 days. The mice were injected intraperitoneally with Ehrlich ascites tumor cells. The mice were injected intraperitoneally with NCPP and CPP 5 times on the 1st day before injection and the next day after injection. The survival rate of mice was calculated. The mice were injected intraperitoneally with NCPP and CPP for 10 days. The phagocytic rate, phagocytic index, levels of H2O2 and NO, peritoneal macrophage (Mφ) phagocytosis rate, spleen NK cell killing rate and T Cell proliferation index. Results: Compared with the control group, NCPP group and CPP group had obvious antitumor activity. The spleen index was higher than 2.0 in peritoneal cavity. The phagocytic rate of Mφ, phagocytic index, production of hydrogen peroxide (H2O2) and nitric oxide NO) significantly increased (P <0.01). The spleen NK cell killing rate was significantly increased (P <0.05). There was no significant difference in spleen T cell proliferation index. In all of the above experiments, there was no significant difference between the NCPP and CPP 2 groups. CONCLUSION: NCPP has the same spleen activation and antitumor activity as CPP. Its mechanism is mainly through the activation of phagocytosis and killing of Mφ and NK cells.