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采用构建猪源MAVS重组质粒pEGFP-MAVS,将其转染PK-15细胞24h后,再感染口蹄疫病毒(MOI=0.1),通过Western blot和real-time PCR检测MAVS融合蛋白表达及对口蹄疫病毒感染宿主细胞的复制和病毒感染滴度。结果显示:重组质粒pEGFP-MAVS能在PK-15细胞中获得表达,并且上调MAVS基因对口蹄疫病毒在宿主细胞中的早期复制有显著抑制作用,病毒的感染滴度也有一定的降低。结果表明:MAVS具有一定的抗病毒作用,此为进一步研究口蹄疫病毒逃逸宿主天然免疫的机制奠定了基础。
The MAVS recombinant plasmid pEGFP-MAVS was constructed and transfected into PK-15 cells for 24 h before infection with foot-and-mouth disease virus (MOI = 0.1). The expression of MAVS fusion protein and its effect on foot-and-mouth disease virus Host cell replication and viral infection titer. The results showed that recombinant plasmid pEGFP-MAVS could be expressed in PK-15 cells and up-regulation of MAVS gene could significantly inhibit the early replication of foot-and-mouth disease virus in host cells, and the virus titer was also decreased. The results showed that: MAVS has a certain antiviral effect, which laid the foundation for further study on the mechanism of natural immunity of foot-and-mouth disease virus escaping host.