作者采用两种化学偶联方法制备了A群脑膜炎球菌多糖(APS)-破伤风类毒素(π)结合物,检测其物理化学及免疫学特性,并用小鼠进行免疫原性研究。偶联法Ⅰ以溴化氰作偶联剂一步完成(简称1.2号),方法Ⅱ先在APS上引入甘氨酸(Gly)或6-氨基正己酸(6-aha)作隔离基团,并以N-乙基-N′-(3-二甲氨基丙基)碳化二亚胺(EDC)作偶联剂,分两步完成APS与π的共价结合,前者为3、4号,后者为5、 The authors used two chemical conjugation methods to prepare group A meningococcal polysaccharide (APS) - tetanus toxoid (π) conjugates, to examine their physico-chemical and immunological properties, and to study their immunogenicity in mice. Coupling method I to cyanogen bromide as a coupling agent in one step (referred to as 1.2), the first method in the introduction of APS glycine (Gly) or 6-aminocaproic acid (6-aha) as a spacer, and N -ethyl-N ’- (3-dimethylaminopropyl) carbodiimide (EDC) as coupling agent, the covalent bonding between APS and π was completed in two steps. The former was 3,4 and the latter was 5,