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经过多年田间和温室接种抗病性鉴定,从(77-5433×中5)杂交组合花药培养后代中选育出一个兼抗大麦黄矮病、条锈、叶锈和秆锈4种小麦主要病害的新种质遗4212。遗4212的体细胞染色体数为42,在减数分裂中期Ⅰ,在几乎所有的花粉母细胞中都可以观察到21个二价体,这说明遗4212是一个在遗传上业已稳定的整倍体材料。对(遗4212×77-5433)F_1代花粉母细胞的观察表明,遗4212可能是含1对外源中间偃麦草染色体的代换系或具较大中间偃麦草染色体片段的易位系。用基因组原位杂交(genomic in situ hybridization,GISH)对遗4212的有丝分裂中期相、减数分裂后期Ⅰ相和(遗4212×77-5433)F_1代花粉母细胞减数分裂中期Ⅰ、后期Ⅰ进行了检测,确证遗4212含1对外源中间偃麦草染色体。这些结果表明,遗4212是一个小麦一中间偃麦草代换系,其抗病性来自其携带的1对中间偃麦草。
After many years of field and greenhouse resistance identification, from the (77-5433 × 5) hybrid combinations of anther culture progeny in a resistance to barley yellow dwarf, stripe rust, leaf rust and stem rust four kinds of wheat disease The new germplasm 4212. The number of somatic chromosomes in the 4212 was 42, and in the metaphase I, 21 bivalents were observed in almost all the pollen mother cells, indicating that 4212 is a genetically stable euploid material. The observation of the F_1 pollen mother cells (QI 4212 × 77-5433) indicated that QI 4212 may be a translocation line with a substitution of the chromosomes of the exogenous Th. Genomic in situ hybridization (GISH) was used to detect the mitotic metaphase, meiotic stage I and meiotic metaphase I and late stage I of F12 pollen mother cell (QI 4212 × 77-5433) The test, indeed confirmed 4212 contains a pair of exogenous Chromosomes. These results show that Yudan 4212 is a wheat-Agropyron exchange line whose disease resistance comes from the 1 pair of Elytrigia intermedium that it carries.