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目的:观察并比较纳米二氧化硅(SiO_2)和微米二氧化硅(SiO_2)颗粒物对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)的毒性作用。方法:将40μg/ml的纳米SiO_2和微米SiO_2颗粒物混悬液分别与HUVECs共培养不同时间(6、12、24、48 h),观察两种颗粒物的毒性作用。检测的毒作用指标有总超氧化物歧化酶(SOD)活性、肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)的释放量。结果:与空白对照组相比,染尘6、12、24、48 h,两组细胞培养液上清中LDH均明显增加(P<0.05);与空白对照组相比,染尘6、12、24、48 h,纳米组细胞培养液上清中TNF-α和IL-6释放量均明显增加(P<0.05);染尘24、48 h,微米组细胞培养液上清中TNF-α和IL-6释放量均明显增加(P<0.05);纳米组和微米组细胞培养液上清中SOD活性先增加后降低(P<0.05)。随染尘时间的延长,两组细胞培养液上清中TNF-α和IL-6释放量均增加,而SOD活性先增加后降低。纳米组细胞培养液上清中LDH、TNF-α及IL-6释放量均明显高于微米组(P<0.05),染尘6、12 h,纳米组SOD活力明显低于微米组(P<0.05),染尘24 h,纳米组SOD活力明显高于微米组(P<0.05)。结论:两种颗粒物均能引起HUVECs损伤,其中纳米SiO_2颗粒物损伤作用较大,两种粒径颗粒物毒性效应存在时间效应。
OBJECTIVE: To observe and compare the toxic effects of nano-silica (SiO 2) and micro-silica (SiO 2) particles on human umbilical vein endothelial cells (HUVECs). Methods: The suspension of 40μg / ml SiO_2 and SiO_2 particles were co-cultured with HUVECs for different time (6, 12, 24, 48 h) respectively. The toxicity of the two kinds of particles was observed. Toxicity indicators were total SOD activity, TNF-α and IL-6 release. Results: Compared with the blank control group, the LDH levels in the supernatant of the two groups of cells were significantly increased at 6, 12, 24 and 48 h (P <0.05). Compared with the blank control group, , 24 and 48 h, the release of TNF-α and IL-6 in the supernatant of the cell culture supernatant increased significantly (P <0.05) (P <0.05). The activity of SOD in the supernatant of the cell culture medium increased first and then decreased (P <0.05) in the nano-group and the micronized group. With the prolongation of the dying time, the release of TNF-α and IL-6 in both cell culture supernatants increased, while the SOD activity increased firstly and then decreased. The release of LDH, TNF-α and IL-6 in the supernatant of the cell culture medium was significantly higher than that of the micronized group (P <0.05), and the activity of SOD in the nano-group was significantly lower than that of the micronized group (P < 0.05). When exposed to dust for 24 h, the activity of SOD in nano-group was significantly higher than that in micro-group (P <0.05). CONCLUSION: Both of the two kinds of particles can cause damage to HUVECs. Among them, the damage of nano-SiO_2 particles is larger, and the toxic effects of two kinds of particles have the time effect.