论文部分内容阅读
为了建立中药闹羊花中Rhodojaponin-III(Rj-III)和Rhodojaponin-VI(Rj-VI)的含量测定方法,本文采用高效液相色谱-蒸发光散射检测方法(HPLC-ELSD)直接测定闹羊花75%丙酮水提取物中Rj-III和Rj-VI的含量。采用Agilent ZORBAX SB-C_18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-水(40∶60)为流动相,流速1 mL/min,柱温30℃,ELSD(Varian 380-L)检测器,雾化器温度55℃,漂移管温度80℃,气体压力为25 psi,载气速度为1.6 L/s,增益为500。结果表明,闹羊花中Rj-III和Rj-VI的含量分别为1.21 mg/g和1.03 mg/g;Rj-III和RjVI回归方程分别为Y=2026.8X-789.2,R~2=0.9979;Y=3777.6X-1849.5,R~2=0.9980;分别在0.4~2.83μg/μL和0.6~4.2μg/μL范围内,进样量与峰面积呈良好的线性关系,该方法简单可行,快速,精密度、重现性和稳定性均较好,可应用于中药闹羊花中Rj-III和Rj-VI的含量测定。
In order to establish a method for the determination of Rhodojaponin-III (Rj-III) and Rhodojaponin-VI (Rj-VI) in Rhizoma Japonica, the direct determination of Rams by sheep by HPLC-ELSD Flower 75% acetone water extract Rj-III and Rj-VI content. The mobile phase consisted of methanol-water (40:60), flow rate 1 mL / min, column temperature 30 ℃ and ELSD (Varian 380-L) using an Agilent ZORBAX SB-C_18 (4.6 mm × 250 mm, The nebulizer temperature was 55 ° C, the drift tube temperature was 80 ° C, the gas pressure was 25 psi, the carrier gas velocity was 1.6 L / s and the gain was 500. The results showed that the contents of Rj-III and Rj-VI were 1.21 mg / g and 1.03 mg / g, respectively. The regression equations of Rj-III and RjVI were Y = 2026.8X-789.2 and R-2 = Y = 3777.6X-1849.5 and R ~ 2 = 0.9980 respectively. There was a good linear relationship between injection volume and peak area in the range of 0.4-2.83μg / μL and 0.6-4.2μg / μL, respectively. The method was simple and feasible, The precision, reproducibility and stability are good, which can be applied to the determination of Rj-III and Rj-VI in Chinese quail blossom.