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目的探讨“温阳益髓”法促进骨髓干细胞定向诱导分化为软骨和成骨细胞治疗兔膝骨关节炎的作用机制。方法选用3月龄的新西兰白兔96只,随机分为空白对照组、骨关节炎模型组、实验对照组和药物实验组。伸直位管型石膏制做兔膝骨关节炎模型,造模成功后给药,按期分批处死,检测TGF、BMP、OCT-4和ABCG2表达水平,进行软骨病理学和细胞基因表达研究。结果 ABCG2、TGF-β和BMP-4模型组、实验对照组和药物实验组与空白对照组相比,差异具有显著统计学意义,ABCG2和OCT-4实验对照组和药物实验组与模型组相比,差异具有显著统计学意义,OCT-4模型组与空白对照组相比,差异具有显著统计学意义,TGF-β药物实验组与模型组相比,差异有显著统计学意义。病理学示模型组软骨退变,细胞形态变异、排列紊乱,软骨基质成分减少。实验对照组和药物实验组均见软骨圆润、光滑,细胞排列整齐,软骨基质成分均匀。结论温阳益髓药物可增强兔机体内生长因子表达,并对机体进行代偿性保护,可以阻止或改善兔膝关节软骨降解和退变。
Objective To investigate the mechanism of “warming yang benefit marrow” method to promote the differentiation of bone marrow stem cells into chondrocytes and osteoblasts for the treatment of osteoarthritis of rabbit knee. Methods 96 New Zealand white rabbits of 3 months old were randomly divided into blank control group, osteoarthritis model group, experimental control group and drug experimental group. Rabbit knee osteoarthritis model was made by extension of position gypsum. After the model was established successfully, the rats were sacrificed in batches. The expression of TGF, BMP, OCT-4 and ABCG2 were detected and the cartilage pathology and gene expression were studied. Results Compared with the blank control group, there was significant difference between ABCG2, TGF-β and BMP-4 model group, experimental control group and drug experimental group and blank control group. There was significant difference between ABCG2, OCT-4 experimental control group and drug experimental group Compared with the control group, the difference was statistically significant. The difference between the TGF-β group and the model group was statistically significant. Pathological model group showed cartilage degeneration, cell morphology variation, disordered arrangement, cartilage matrix components decreased. Experimental control group and drug experimental group were seen round cartilage smooth, smooth arrangement of cells, cartilage matrix composition. Conclusion WEN can enhance the expression of growth factor in rabbits and compensatory protection to the body, which can prevent or ameliorate the degeneration and degeneration of rabbit articular cartilage.