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目的:建立益肾补骨口服液中2,3,5,4′-四羟基二苯乙烯-2-O-β-D·葡萄糖苷的含量测定方法。方法:采用Thermo Gold C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(20:80);流速1.0 ml·min~(-1);检测波长为320 nm。结果:2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在7.5～62.8μg·ml~(-1)的范围内线性关系良好,r=0.999 7,平均回收率102.3%,RSD=0.8%。结论:该方法简便,可靠,可用于益肾补骨口服液的质量控制。
Objective: To establish a method for the determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D·glucoside in Yishenbugu oral liquid. METHODS: The Thermo Gold C 18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-water (20:80). The flow rate was 1.0 ml·min -1 and the detection wavelength was 320 nm. Results: The linear relationship of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside was good in the range of 7.5-62.8 μg·ml -1 , r=0.999 7 The average recovery rate was 102.3% with RSD = 0.8%. Conclusion: This method is simple, reliable and can be used for the quality control of Yishen Bugu oral liquid.