论文部分内容阅读
目的探讨不同剂量曲尼司特(TNL)对环孢素A(CsA)慢性肾毒性大鼠肾脏TGF-β/Smad通路的的影响。方法雄性SD大鼠34只,随机分成正常对照组、模型组、TNL低剂量治疗组、TNL中剂量治疗组、TNL高剂量治疗组、安博维治疗组。采用低盐饮食加CsA20mg/(kg·d)灌胃的方法建立环孢素A慢性肾毒性大鼠模型。用RT-PCR和免疫组织化学检测不同剂量TNL对大鼠肾脏TGF-β1、Smad3、Smad7的影响。结果TNL能下调CsA慢性肾毒性大鼠肾组织中TGF-β1、Smad3的mRNA水平及在肾脏的表达,上调Smad7的mRNA水平及在肾脏的表达。结论在CsA慢性肾毒性大鼠模型中,TNL可能通过调节TGF-β/Smad通路而发挥抗纤维化的作用,从而减缓CsA慢性肾毒性的进展。
Objective To investigate the effects of different doses of tranilast (TNL) on the TGF-β / Smad pathway in the kidney of chronic nephrotoxic rats with cyclosporin A (CsA). Methods Thirty-four male Sprague-Dawley rats were randomly divided into normal control group, model group, TNL low-dose treatment group, TNL middle-dose treatment group, TNL high-dose treatment group and Ambroxol treatment group. A chronic nephrotoxic rat model of cyclosporine A was established by intragastric administration of low-salt diet plus CsA 20 mg / (kg · d). The effects of different doses of TNL on the expression of TGF-β1, Smad3 and Smad7 in rat kidney were detected by RT-PCR and immunohistochemistry. Results TNL could down-regulate the mRNA and protein levels of TGF-β1 and Smad3 in the kidney of CsA-induced chronic nephrotoxic rats, and upregulate the expression of Smad7 and its expression in the kidney. Conclusion In the chronic nephrotoxic rat model of CsA, TNL may play an anti-fibrotic role by regulating the TGF-β / Smad pathway, thereby slowing the progression of chronic nephrotoxic CsA.