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选取暖温带海洋生态环境生物修复的首选物种——铜藻(Sargassum horneri)为原料进行生物乙醇的制备,以稀硫酸水解后的还原糖收率为响应值,考察水解温度、液固比、水解时间和w(H2SO4)等参数对水解效率的影响.为优化稀酸水解铜藻预处理的工艺条件,在单因素试验的基础上,利用Box-Benhnken中心组合设计法和响应面分析法,建立稀酸预处理工艺参数的回归模型,并与酶水解及发酵相结合验证了铜藻稀酸预处理效果.结果表明:①稀酸水解铜藻的最优工艺参数.水解温度为120℃,液固比为20∶1,水解时间为2.00 h,w(H2SO4)为4.50%.②稀酸水解铜藻过程中各影响因素之间存在交互作用,水解时间和w(H2SO4)的非线性作用显著.③对经最佳稀酸预处理工艺处理后的铜藻粉进行酶水解,其还原糖收率为44.05%,是未预处理下的8.14倍,并且后续进行发酵后,乙醇产率达7.80%,是未预处理下的2.00倍.表明铜藻是一种潜在的生物乙醇原料,稀酸预处理方法对铜藻生物乙醇的制备行之有效.
The best species of bioremediation in the warm temperate marine environment was selected, Sargassum horneri was used as raw material to prepare bioethanol. The yield of reducing sugar after dilute sulfuric acid hydrolysis was used as the response value. The effects of hydrolysis temperature, liquid to solid ratio, hydrolysis Time and w (H2SO4) on the hydrolysis efficiency.In order to optimize the pretreatment conditions of dilute-acid hydrolyzed copper algae, based on the single-factor experiment and the Box-Benhnken central composite design and response surface analysis The results showed that: (1) The optimum technological parameters of dilute acid hydrolysis of copper algae were as follows: the hydrolysis temperature was 120 ℃, the liquid The solid ratio is 20: 1, the hydrolysis time is 2.00 h, and the w (H2SO4) is 4.50% .②The interaction between the various factors in the process of dilute acid hydrolysis of copper algae is obvious. The nonlinear effect of hydrolysis time and w (H2SO4) .③After enzymatic hydrolysis of the copper algae powder treated by the optimal dilute acid pretreatment process, the yield of reducing sugar was 44.05%, 8.14 times higher than that of the untreated, and the yield of ethanol reached 7.80 %, 2.00 times without pretreatment Sargassu is a potential feedstock bioethanol, dilute acid pretreatment process for the preparation of bioethanol Sargassu effective.