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目的:建立一种操作简便、周期短且模拟性好的溃疡性结肠炎相关性结直肠癌动物研究模型。方法:20只SD大鼠采用完全随机方法分为2个实验组。模型组以含2%右旋葡聚糖苷钠(DSS)的水溶液自由饮用1周,对照组则饮用清洁水;次周,模型组腹腔注射氧化偶氮甲烷(AOM)1次(剂量为15mg/kg),对照组注射等量的生理盐水;第3周,重复注射1次;16周后,所有大鼠解剖观察。结果:模型组大鼠6只肉眼就可见13颗肿瘤生成,肿瘤发生率为60%(6/10)。模型组〔(0.812 3±0.042 3)g〕大鼠脾脏质量与对照组〔(0.761 9±0.035 3)g〕差异无统计学意义,P>0.05;而胸腺质量相差明显〔模型组为(0.448 0±0.049 9)g,对照组为(0.320 7±0.016 0)g〕,P<0.05。结论:DSS联合AOM能够有效诱导SD大鼠溃疡性结肠炎相关性结直肠癌的发生,并具较好模拟性,且操作也较简便、周期短,对研究肿瘤发生与药物应用不失为一良好模型。
OBJECTIVE: To establish an animal model of colorectal cancer associated with ulcerative colitis with simple, short cycle and good simulation. Methods: Twenty SD rats were randomly divided into two groups. The model group was given free drinking water for 1 week with 2% dextran sodium (DSS) aqueous solution and the control group drinking clean water. In the next week, the model group was given intraperitoneal injection of AOM (15mg / kg). The control group was injected with the same amount of saline. The third week was repeated once. After 16 weeks, all the rats were dissected. Results: Totally 13 tumors were found in the 6 eyes of the model group, with a tumor incidence of 60% (6/10). There was no significant difference in the spleen mass between the model group [(0.812 3 ± 0.042 3) g] and the control group (0.761 9 ± 0.035 3) g, P> 0.05; however, the difference of the thymus mass was significant 0 ± 0.049 9) g, and the control group was (0.320 7 ± 0.016 0) g], P <0.05. Conclusion: DSS combined with AOM can induce ulcerative colitis-associated colorectal cancer in SD rats effectively, and has better simulation, more convenient operation and shorter cycle, so it is a good model to study tumorigenesis and drug application .