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光学显微镜在综合大学和师范院校生物系及农、林、医院校中,使用非常广泛。其一般使用方法为人们所熟知,但在使用时,显微镜是否处于最佳工作状态,能否发挥最佳效能却是常被忽视的问题。光学显微镜的光学部件包括物镜、目镜和聚光器三部分。它们都有各自的技术参数。涉及物镜的主要有分辨率、焦深(景深)和覆盖差,与物镜和聚光器有关的有数值孔径,与物镜和目镜有关的有放大率。
Optical microscopes are widely used in the biology departments of universities and normal universities and in agriculture, forestry and medical schools. The general use of the method is well known, but in use, the microscope is in the best working condition, can play the best performance is often overlooked. Optical parts of the optical microscope includes the objective lens, eyepiece and condenser three parts. They have their own technical parameters. The main objectives involved in the resolution, depth of focus (depth of field) and coverage is poor, and the objective lens and condenser are related to the numerical aperture, and objective and eyepiece are related to the magnification.