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目的探讨熊果酸对人高转移卵巢癌细胞HO-8910PM侵袭转移的影响及其可能的机制。方法以不同浓度的熊果酸处理高转移卵巢癌细胞HO-8910PM后,采用MTT法检测其对细胞的生长抑制作用,应用Transwell小室进行人工重组基底膜侵袭和运动实验,观察熊果酸对HO-8910PM细胞侵袭和转移的影响,明胶酶谱法检测HO-8910PM细胞基质金属蛋白酶-2-9、(MMP-2、MMP-9)的活性变化,Westen blot方法检测细胞中MMP-2和MMP-9蛋白表达变化。结果熊果酸处理后卵巢癌细胞生长受到抑制,且作用呈时效、量效依赖关系,熊果酸能抑制卵巢癌细胞HO-8910PM侵袭和运动能力,各实验组与对照组比较差异有统计学意义(P<0.01,P<0.05);熊果酸能抑制卵巢癌细胞HO-8910PM明胶酶活性(P<0.01),抑制卵巢癌HO-8910PM细胞MMP-2和MMP-9蛋白表达,各实验组与对照组比较差异有统计学意义(P<0.05)。结论熊果酸能抑制卵巢癌细胞侵袭和转移,其机制可能与抑制MMP-2和MMP-9酶活性和蛋白表达有关。
Objective To investigate the effect of ursolic acid on the invasion and metastasis of human highly metastatic ovarian cancer cell line HO-8910PM and its possible mechanism. Methods After treated with ursolic acid at different concentrations for HO-8910PM cells with high metastatic potential, MTT assay was used to detect the inhibitory effect on the cell growth. Transwell chambers were used to investigate the invasion and migration of human ovarian cancer cell line HO-8910PM. -8910PM cell invasion and metastasis. The activity of MMP-2 and MMP-9 in HO-8910PM cells was detected by gelatin zymography. The expressions of MMP-2 and MMP-9 -9 protein expression changes. Results The ursolic acid treatment inhibited the growth of ovarian cancer cells in a time-dependent and dose-dependent manner. Ursolic acid could inhibit the invasion and exercise capacity of ovarian cancer cells HO-8910PM. The differences between the experimental and control groups were statistically significant (P <0.01, P <0.05). UA inhibited the activity of HO-8910PM in ovarian cancer cells (P <0.01) and inhibited the expression of MMP-2 and MMP-9 in HO-8910PM cells. The difference between the two groups was statistically significant (P <0.05). Conclusion UA can inhibit the invasion and metastasis of ovarian cancer cells. The mechanism may be related to the inhibition of MMP-2 and MMP-9 activity and protein expression.