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选用芜菁种子,使用20%PEG-6000模拟干旱胁迫,1.0μmol/L高铁血红素和200μmol/L硝普钠分别作为外源CO和NO供体,研究外源CO、NO对干旱胁迫下芜菁种子萌发的影响。结果表明,200μmol/L硝普钠处理后,可以显著缓解干旱胁迫对芜菁种子造成的伤害,使种子发芽率、发芽势分别提高26.19%、117.39%,处理第5天,与20%PEG-6000处理相比,添加硝普钠处理芜菁胚根长度增加94.39%,种子可溶性糖、可溶性蛋白、脯氨酸、含水量分别提高31.58%、73.29%、64.46%、34.47%,超氧化物歧化酶活性提高45.60%,丙二醛含量降低19.77%;1.0μmol/L高铁血红素处理后,同样对芜菁种子所受干旱胁迫损伤有显著的缓解效应,使种子发芽率、发芽势分别提高30.63%、107.14%,处理的第5天,与20%PEG-6000处理相比,添加高铁血红素处理芜菁胚根长度增加83.70%,种子可溶性糖、可溶性蛋白、脯氨酸、含水量分别提高39.58%、70.55%、59.78%、22.62%,超氧化物歧化酶活性提高43.00%,丙二醛含量降低21.51%。说明高铁血红素和硝普钠的添加,可以通过提高受胁迫种子可溶性糖、可溶性蛋白、脯氨酸含量,从而降低渗透势,提高抗氧化物酶活性,降低过氧化物含量,从而加速种子萌发。
Using turnip seeds, 20% PEG-6000 simulated drought stress, 1.0μmol / L hematin and 200μmol / L sodium nitroprusside were used as exogenous CO and NO donors, respectively. Effect of Jing seed germination. The results showed that the treatment of 200 μmol / L sodium nitroprusside could significantly alleviate the damage caused by drought stress on the turnip seeds, the seed germination rate and germination potential were increased by 26.19% and 117.39%, respectively. On the fifth day and 20% PEG- 6000 treatment, the length of root of turnip increased by 94.39% and the content of soluble sugar, soluble protein, proline and water content increased by 31.58%, 73.29%, 64.46%, 34.47%, respectively, Enzyme activity increased by 45.60% and malondialdehyde content decreased by 19.77%. After treated with 1.0μmol / L hemin, the germination rate and germination potential of turnip seeds were also significantly reduced by 30.63 % And 107.14%, respectively. Compared with 20% PEG-6000 treatment, the radicle length increased by 83.70% and the content of soluble sugar, soluble protein, proline and water content increased 39.58%, 70.55%, 59.78% and 22.62%, SOD activity increased 43.00% and malondialdehyde content decreased 21.51%. The results showed that the addition of hemin and sodium nitroprusside could accelerate the seed germination by increasing the content of soluble sugar, soluble protein and proline in stressed seeds, thereby reducing the osmotic potential, increasing the activity of antioxidant enzymes and reducing the peroxide content .