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测定5-氟脲嘧啶(5-Fu)血药浓度的方法有生物法、同位素标记法、分光光度法、气相法及高效液相法。本文采用氟离子选择电极对5-Fu 的血浓度作了初步测定,测定灵敏度为0.19μgF/ml,即相当于5-Fu 1.3μg/ml,血浓度的线性范围为10～90μg/ml血浆。1.直接燃烧法:在无灰滤纸上滴加400μl家兔血浆,吹干,用氧瓶燃烧后吸收于加有50μl3%H_2O_2的10ml重蒸水(或 pH 为5～6的醋酸缓冲液)中,用氟电极及带有硝酸钾外盐桥的饱和甘汞电极测定电位。2.提取燃烧法:取家兔血浆1 ml,加200μlpH
Determination of 5 - fluorouracil (5-Fu) blood concentration methods are biological method, isotope labeling method, spectrophotometry, gas phase and high performance liquid phase method. In this paper, fluoride ion selective electrode 5-Fu blood concentration was initially determined, the measured sensitivity of 0.19μgF / ml, which is equivalent to 5-Fu 1.3μg / ml, the linear range of the blood concentration of 10 ~ 90μg / ml plasma. 1. Direct combustion method: on the ashless filter paper dropping 400μl rabbit plasma, dry, oxygen cylinder flasked with 50μl3% H_2O_2 added 10ml of distilled water (or pH 5 to 6 acetate buffer) , The potential was measured with a fluoride electrode and a saturated calomel electrode with a salt bridge of potassium nitrate. 2. Extraction combustion method: take rabbit plasma 1 ml, add 200μlpH