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目的:构建过氧化物酶体增殖物激活受体γ(PPARγ)基因沉默的高糖致伤血管内皮细胞株并研究其增殖活性。方法:应用RNA干扰技术沉默PPARγ基因的表达,用高糖培养基持续培养致伤人脐静脉内皮细胞(HUVEC),采用细胞连续生长计数及MTT比色法测定细胞增殖活性。结果:获得PPARγ沉默的HUVEC细胞株,相对表达量仅有出发细胞的23.56%;复制了高糖致伤模型,其SA-β-半乳糖苷酶明显增高(P<0.01),而NO水平明显下降(P<0.01)。与未经高糖处理的PPARγ基因沉默HUVEC相比,高糖致伤的PPARγ基因沉默组细胞增殖活性明显降低(P<0.05)。结论:成功构建了PPARγ基因沉默的高糖致伤血管内皮细胞株,并提示PPARγ表达下调明显影响了高糖致伤HUVEC细胞的分裂增殖能力,这对进一步研究PPARγ在糖尿病合并急性冠状动脉综合征中的作用机制有重要意义。
Objective: To construct peroxisome proliferator - activated receptor γ (PPARγ) gene silenced hyperglycemic vascular endothelial cell line and study its proliferative activity. Methods: The expression of PPARγ gene was silenced by RNA interference technique. Human umbilical vein endothelial cells (HUVECs) were cultured with high glucose medium continuously. Cell proliferation was measured by cell growth counting and MTT assay. Results: The relative expression level of HUVEC cells transfected with PPARγ was only 23.56% of that of the starting cells. The model of injury induced by high glucose was significantly increased (P <0.01) and the level of NO was significantly higher Decreased (P <0.01). The proliferation of PPARγ gene silencing group was significantly lower than that of HUVEC without PPARγ gene silencing (P <0.05). CONCLUSION: A high glucose-injured vascular endothelial cell line with PPARγ gene silencing has been successfully constructed. It is suggested that the downregulation of PPARγ significantly affects the ability of HUVEC cells to divide and proliferate under high glucose, which is of great value in the further study of PPARγ in diabetic patients with acute coronary syndrome In the mechanism of action is of great significance.