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目的测定不同品种桑叶在不同采收时间总多酚及绿原酸、芦丁、安息香酸和黄芪苷4种主要酚类单体化合物的量,初步探讨桑叶酚类物质的变化规律。方法采用Folin-Phenol法测定桑叶中总酚的量,采用HPLC法测定绿原酸、芦丁、安息香酸和黄芪苷的量。结果 6个品种桑叶总多酚量在4~10月连续7个月内变化的趋势基本相同。各品种中13种酚类单体物质的变化比较复杂:同一品种桑叶中不同酚类物质变化不一样,不同品种桑叶中相同酚类物质变化也不一样。结论不同品种桑叶的酚类单体成分的组成及其变化规律具有品种特异性,在相同栽培条件及气候条件下,桑叶中酚类物质的量在不同品种之间有差异,但在所有品种中多酚的量均以绿原酸最高,其次为芦丁,再次为安息香酸和黄芪苷。
OBJECTIVE To determine the contents of total polyphenols, chlorogenic acid, rutin, benzoic acid and astragaloside in different mulberry leaves at different harvesting times, and to explore the variation regularity of phenols in mulberry leaves. Methods Folin-Phenol method was used to determine the amount of total phenols in mulberry leaves. The contents of chlorogenic acid, rutin, benzoic acid and astragaloside were determined by HPLC. Results The trend of total polyphenols content in mulberry leaves of 6 cultivars varied from April to October for 7 consecutive months. The changes of 13 kinds of phenolic monomers in each variety were complicated: the changes of different phenols in the same variety of mulberry leaves were different, and the changes of the same phenols in different varieties of mulberry leaves were also different. Conclusion The composition and variation of phenolic monomer composition in different varieties of mulberry leaves are species-specific. Under the same cultivation conditions and climatic conditions, the amount of phenolic compounds in mulberry leaves varies among different varieties. However, The amount of polyphenols in the varieties are the highest chlorogenic acid, followed by rutin, again for the benzoic acid and astragaloside.