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冬葵(Malva verticillata L.)是一种具有很高药用价值的蒙药,实验以冬葵叶片和茎段为外植体诱导愈伤组织,为冬葵的快繁和细胞培养奠定基础。实验结果如下:冬葵叶片和茎段最适消毒条件是0.1%升汞分别消毒50s、2.5min;叶片和茎段诱导愈伤组织阶段最适培养基相同,均为MS+蔗糖(30g/L)+琼脂(7g/L)+NAA(0.4mg/L)+6-BA(3mg/L)+抗坏血酸(600mg/L)。
Malva verticillata L. is a kind of Mongolian medicine with high medicinal value. The experiment used mallow leaves and stems as explants to induce callus, which laid the foundation for the rapid propagation and cell culture of mallow. The results showed as follows: The optimum disinfection conditions for leaf and stem of mallow were: 0.1% mercuric chloride was disinfected for 50s and 2.5min respectively; MS and sucrose (30g / L) were the best medium for inducing callus in leaves and stems, + Agar (7 g / L) + NAA (0.4 mg / L) + 6-BA (3 mg / L) + ascorbic acid (600 mg / L).