论文部分内容阅读
目的:探索中药草苁蓉提取物对β淀粉样蛋白(β-AP)所致AD模型大鼠的脑内乙酰胆碱(Ach)含量、乙酰胆碱转移酶(ChAT)及乙酰胆碱酯酶(AchE)活性的影响。方法:将60只健康SD大鼠随机分为正常组、假手术组、模型组和中药高、中、低剂量组。采用Aβ1-40建立AD模型,中药组予以草苁蓉提取物按高、中、低3种不同剂量灌胃治疗,于治疗后检测各组海马和皮质部位Ach、ChAT及AchE的含量或活性。结果:与模型组相比,中药组Ach含量和ChAT活性升高(P<0.01),而TchE活性降低(P<0.01)。结论:中药草苁蓉提取物能提高AD模型大鼠脑内ChAT活性,抑制AchE活性,从而增加脑内Ach含量。
OBJECTIVE: To investigate the effects of Herba Sigranae extract on the contents of acetylcholine (Ach), acetylcholine transferase (ChAT) and acetylcholinesterase (AchE) in brains of AD rats induced by β-amyloid protein (β-AP) . Methods: Sixty healthy Sprague-Dawley rats were randomly divided into normal group, sham operation group, model group and high, middle and low dose groups of traditional Chinese medicine. Aβ1-40 was used to establish an AD model. The traditional Chinese medicine group was given a high-, middle-, and low-dose of Herba Siebae extract by intragastric administration. The content or activity of Ach, ChAT, and AchE in the hippocampus and cortex of each group was determined after treatment. Results: Compared with the model group, the Ach content and ChAT activity in the Chinese medicine group were increased (P<0.01), while the TchE activity was decreased (P<0.01). Conclusion: Herba Siegesbeckiae extract can increase the ChAT activity in the brain of AD rats, inhibit the AchE activity, and increase the Ach content in the brain.