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目的:应用组织芯片技术研究间变性大细胞淋巴瘤(ALCL)中ALK蛋白的表达及其与患者年龄和预后的关系。方法:建立包含30例ALCL及2例对照组织共96点阵的组织芯片,采用免疫组织化学SP法检测ALK蛋白表达,并应用SPSS软件进行统计学分析。结果:(1)ALK蛋自在2例皮肤原发ALCL均为阴性,28例系统性ALCL中有20例(71.4%)阳性,主要为细胞浆和/或细胞核棕黄色。(2)ALK蛋白阳性组的年龄低于ALK蛋白阴性组的年龄,两者差异具有显著性(P<0.05)。(3)ALK蛋白阳性组的预后好于ALK蛋白阴性组的预后,两者差异具有显著性(P<0.05)。结论:ALK蛋白的表达在ALCL中有很高的发生率,尤其是低年龄组的患者,它可以成为ALCL的诊断、鉴别诊断和判断预后的一个独立重要指标。
Objective: To study the expression of ALK protein in patients with anaplastic large cell lymphoma (ALCL) and its relationship with age and prognosis of patients by tissue microarray. Methods: Tissue microarrays containing 96 ALCLs in 30 cases and 30 controls were established. The expression of ALK protein was detected by immunohistochemical SP method and analyzed by SPSS software. Results: (1) ALK eggs were negative in 2 cases of primary skin ALCL. Twenty cases (71.4%) were positive in 28 cases of systemic ALCL, mainly cytoplasm and / or nucleus brown. (2) The age of ALK protein positive group was lower than that of ALK protein negative group, the difference was significant (P <0.05). (3) The prognosis of ALK positive group is better than that of negative ALK negative group (P <0.05). Conclusion: The expression of ALK protein is highly expressed in ALCL, especially in patients with lower age. It may become an independent and important indicator of diagnosis, differential diagnosis and prognosis of ALCL.