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本研究应用粘液组化方法观察了直肠癌旁TM的范围,应用免疫组化方法观察了MTSI基因产物在直肠癌旁TM的表达,并与正常粘膜及痛组织进行对照。直肠癌手术标本81例,男51例,女30例,年龄53±11.4岁。按统一组织学分类为乳头状腺癌30例,管状腺癌30例,粘液癌18例,未分化癌1例。收集标本后,经10%的中性福尔马林固定后,沿肠管纵向自癌肿向两断端每0.5cm等长取材,石蜡包埋,制成5μm厚切片。每例切片均作HID-AB粘液染色,免疫组化染色,用PBS液代替一抗作阴性对照,用已知阳性标本作阳性对照,操作按说明书进行。结果:正常直肠粘膜(NM)、移行粘膜(TM)及癌组织中均有MTSI基因产物表达,表达率逐渐下降,3组问差异显著(P<0.05)。在癌旁组织及癌组织,MTSI基因产物均位于细胞浆,未见细胞核及细胞膜阳性,阳性细胞呈弥漫性、局灶性或散在性分布。MTSI阴性的直肠癌病例,其TM范围均明显高于MTSI阳性的直肠癌病例(P<0.05)。
In this study, the range of TM adjacent to rectal cancer was observed by mucohistochemistry. The expression of MTSI gene product adjacent to rectal cancer was observed by immunohistochemistry and compared with normal mucosa and pain. 81 rectal cancer surgical specimens, 51 males and 30 females, age 53 ± 11.4 years old. According to histological classification, there were 30 cases of papillary adenocarcinoma, 30 cases of tubular adenocarcinoma, 18 cases of mucinous carcinoma, and 1 case of undifferentiated carcinoma. After the specimens were collected, they were fixed with 10% neutral formalin, drawn along the length of the intestine from the cancer to the two broken ends every 0.5 cm and were embedded in paraffin, and were made into 5 μm thick sections. Each section was stained with HID-AB mucus. Immunohistochemical staining was performed. PBS was used instead of primary antibody as a negative control. Known positive samples were used as positive controls. Results: MTSI gene products were expressed in normal rectal mucosa (NM), transitional mucous membrane (TM) and cancer tissues. The expression rate gradually decreased. The differences among the three groups were significant (P<0.05). In paracancerous tissues and cancerous tissues, MTSI gene products are located in cytoplasm, no positive nucleus and cell membrane, and positive cells are diffuse, focal or scattered distribution. MTSI-negative cases of rectal cancer were significantly higher in TM range than those in MTSI-positive rectal cancer (P<0.05).