Six soil enzymes (invertase, acid phosphatase, proteinase, catalase, peroxidase and polyphenoloxidase ) were chosen for investigation under different spruce forests with restoration ages of 4,10,16 years and an old-growth spruce forest over 400 years old in the eastern Qinghai-Tibet Plateau, China. Results showed that the activities of invertase, phosphatase, proteinase, catalase and peroxidase decreased in newly restored forests except for pholyphenoloxidase. With the development of forests after restoration, the activities of invertase, acid phosphadase, proteinase increased gradually. Our study also indicated that the soil enzyme activities were associated with surface soils and decreased with depths. This result suggested that in the earlier restoration stage the application of organic fertilizer may be more effective by surface addition to soils than deep addition. Six soil enzymes (invertase, acid phosphatase, proteinase, catalase, peroxidase and polyphenoloxidase) were chosen for investigation under different spruce forests with restoration ages of 4,10,16 years and an old-growth spruce forest over 400 years old in the eastern Qinghai -Tibet Plateau, China. Results showed that the activities of invertase, phosphatase, proteinase, catalase and peroxidase decreased in newly restored forests except for pholyphenoloxidase. With the development of forests after restoration, the activities of invertase, acid phosphadase, proteinase increased gradually. Our study also indicates that the soil enzyme activities were associated with surface soils and decreased with depths. This result suggests that in the earlier restoration stage the application restoration of organic fertilizer may be more effective by surface addition to soils than deep addition.