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【目的】研究棉花细胞质雄性不育的相关线粒体基因。【方法】利用线粒体基因atp A、atp6、atp9、cob、coxⅠ、coxⅡ、coxⅢ、nad3、nad6、nad9探针,对哈克尼西棉细胞质雄性不育系S、保持系F、杂种一代H进行限制性片段长度多态性分析。【结果】atp A、coxⅢ、nad3、nad6在3个材料间具有多态性。atp A、nad6基因探针与所有酶的杂交结果均显示出多态性,且在不育系与杂种一代中表现一致,而在保持系中差异明显。atp A/Eco RⅠ在3个材料中的杂交结果均显示2条带,其中1条2.2 kb的杂交带在3个材料中相同,另一条在不育系和杂种一代中大小为3.2 kb,而在保持系中为4.8 kb;atp A/PstⅠ杂交结果中,在不育系和杂种一代中的条带大小为17.0kb,而在保持系中为10.2 kb。nad6探针与4个酶的杂交结果均为不育系和杂种一代比保持系多1~2条杂交带。coxⅢ/Eco RⅠ在3个材料中均有1条2.5 kb的杂交带,但在保持系与杂种一代中比不育系多1条1.7 kb的弱带。nad3/Bam HⅠ的杂交结果在不育系与保持系中表现一致,而在杂种一代中缺少1条9.5 kb的杂交带。【结论】推测atp A、nad6基因参与调控不育系的形成,而coxⅢ、nad3可能受到恢复系核基因的调控,在不育系育性恢复过程中发挥较为重要的作用。
【Objective】 The purpose of this study was to investigate the mitochondrial genes related to cytoplasmic male sterility in cotton. 【Method】 The cytoplasmic male sterile lines Hu, Neisseria gonorrhoeae, maintainer line F and hybrid H of mitochondrial DNA were amplified by using atp A, atp6, atp9, cob, coxⅠ, coxⅡ, coxⅢ, nad3, nad6 and nad9 probes Restriction fragment length polymorphism analysis. 【Result】 Atp A, coxⅢ, nad3 and nad6 were polymorphic among the three materials. The hybridization results of atp A and nad6 gene probes with all the enzymes showed polymorphism, and they were consistent in CMS and F1 hybrids, but significantly different in maintainer lines. The hybridization results of atp A / Eco RI in the three materials showed two bands, of which one 2.2 kb hybrid band was the same among the three materials and the other was 3.2 kb in the male sterile and hybrid F1 4.8 kb in the maintainer line, 17.0 kb in the atp A / Pst I hybridization, and 10.2 kb in the maintainer line. The results of hybridization between nad6 probe and four enzymes were all from 1 to 2 bands in CMS and F1 hybrids. The coxⅢ / EcoRI had a 2.5kb hybridization band in each of the three materials, but one 1.7kb weak band in the maintainer and hybrid generations more than the male sterile line. The hybridization results of nad3 / Bam HI were consistent in CMS and maintainer lines, while one hybridization band lacking 9.5 kb was found in hybrids. 【Conclusion】 It is speculated that atp A and nad6 genes are involved in the regulation of CMS formation. However, cox Ⅲ and nad3 may be regulated by restorer line nuclear genes and play an important role in the fertility restoration of CMS lines.