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目的:观察荞麦花叶总黄酮(TFBFL)对阿霉素致心力衰竭大鼠的保护作用并分析其可能机制。方法:SD大鼠随机分为正常对照组、模型对照组、荞麦花叶总黄酮低、中、高剂量组(100,200,400 mg·kg-1)及卡托普利组(10 mg·kg-1)。采用腹腔注射阿霉素建立大鼠心衰模型,于造模第4周各组同时灌胃给予治疗药物,1次/d,连续3周。末次给药后测定各组大鼠心功能指标;放射免疫法测定血清心钠素(ANP)、脑钠素(BNP)水平;ELISA法检测血清中一氧化氮(NO)、一氧化氮合酶(NOS)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平。结果:与正常对照组比较,模型对照组大鼠心功能各项指标如左室收缩压(LVSP)、左室舒张末压(LVEDP)、左室内压最大上升、下降速度(±dp/dtmax)及血清中ANP,BNP,NO,NOS,IL-1β,IL-6及TNF-α水平均出现显著升高(P<0.05,P<0.01);与模型对照组比较,TFBFL各剂量组大鼠上述心功能各项指标及血清中各指标均出现不同程度的降低(P<0.05,P<0.01)。结论:TFBFL可改善阿霉素致心衰大鼠心功能,其机制可能与其降低血清中ANP,BNP,NO,NOS,IL-1β,IL-6及TNF-α水平相关。
Objective: To observe the protective effect of TFBFL on the doxorubicin-induced heart failure rats and analyze its possible mechanism. Methods: SD rats were randomly divided into normal control group, model control group, low, medium and high dose buckwheat leaf flavonoids (100,200,400 mg · kg-1) and captopril group (10 mg · kg-1) . Adriamycin was intraperitoneally injected to establish a rat model of heart failure. All rats in the 4th week of the model were given gavage once a day for 3 weeks. The cardiac function of rats in each group was measured after the last administration, serum ANP and BNP levels were measured by radioimmunoassay, serum nitric oxide (NO), nitric oxide synthase (NOS), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) Results: Compared with normal control group, the indexes of left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), maximum left ventricular pressure (± dp / dtmax) (P <0.05, P <0.01). Compared with the model control group, the levels of ANP, BNP, NO, NOS, IL-1β, IL-6 and TNF- The indicators of cardiac function and serum indicators showed varying degrees of reduction (P <0.05, P <0.01). CONCLUSION: TFBFL can improve cardiac function in rats with adriamycin-induced heart failure and its mechanism may be related to the decrease of serum ANP, BNP, NO, NOS, IL-1β, IL-6 and TNF-α.