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探讨TGF-β/Smad信号通路在艾滋病患者CD8+T细胞功能损害中的作用。在HIV感染者(CD4+T细胞计数≥200个/μL)和艾滋病病人(CD4+T细胞计数<200个/μL)中各抽取30例,另以30例健康成人为对照。取研究对象的外周静脉血,流式细胞术检测CD8+T细胞CD28分子和磷酸化Smad2/3蛋白(p-Smad2/3),酶联免疫吸附测定法(ELISA)检测血浆中TGF-β1、IFN-γ和TNF-α。健康人、HIV感染者和AIDS病人CD8+CD28+双阳性细胞占CD8+T细胞的比例分别为47.75%、47.40%和36.20%(中位数),AIDS病人低于正常人和HIV感染者(P<0.05);p-Smad2/3阳性CD8+T细胞占CD8+T细胞的比例分别为79.15%、55.60%和60.60%(中位数),HIV感染者和AIDS病人低于健康人(P<0.05);血浆TNF-α分别为(147.92±1.47)pg/mL、(60.04±1.78)pg/mL和(54.43±2.03)pg/mL,HIV感染者和AIDS病人低于健康人(P<0.05);血浆TGF-β1和IFN-γ的组间差异无统计学意义。TGF-β/Smad信号通路可能参与了艾滋病患者CD8+T细胞的异常激活与功能损害。
To investigate the role of TGF-β / Smad signaling pathway in the functional impairment of AIDS patients with CD8 + T cells. Thirty cases were selected from each of HIV infected persons (CD4 + T cell count ≥200 / μL) and AIDS patients (CD4 + T cell count <200 cells / μL), and the other 30 healthy adults as controls. Peripheral venous blood was collected from peripheral blood mononuclear cells (PBMCs) of CD8 + T cells and phosphorylated Smad2 / 3 protein (p-Smad2 / 3) were detected by flow cytometry. The levels of TGF- IFN-γ and TNF-α. The proportion of CD8 + CD28 + double positive cells to CD8 + T cells in healthy people, HIV infected persons and AIDS patients was 47.75%, 47.40% and 36.20% respectively (median), while those in AIDS patients were lower than those in normal people and HIV infected persons <0.05). The proportion of p-Smad2 / 3 positive CD8 + T cells to CD8 + T cells was 79.15%, 55.60% and 60.60%, respectively. The median of HIV-infected and AIDS patients was lower than that of healthy people (P < 0.05). The plasma levels of TNF-α were (147.92 ± 1.47) pg / mL, (60.04 ± 1.78) pg / mL and (54.43 ± 2.03) pg / mL, respectively. ). There was no significant difference between the two groups in plasma TGF-β1 and IFN-γ. TGF-β / Smad signaling pathway may be involved in the abnormal activation and dysfunction of CD8 + T cells in AIDS patients.