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目的：研究精氨加压素（４－８）（ＡＶＰ（４－８））对大鼠脑内促细胞分裂素活化的蛋白激酶（ＭＡＰＫ）活性变化的影响．方法：大鼠皮下注射ＡＶＰ（４－８）后，通过柱分离后测定脑组织匀浆液中ＭＡＰＫ对其特异性底物髓脂质碱性蛋白（ＭＢＰ）的磷酸化活性．结果：ＡＶＰ（４－８）刺激的大鼠海马和大脑皮层中的４４ｋＤａＭＡＰＫ活性显著增高．这种活性增高被ＡＶＰ（４－８）的拮抗剂ＺＤＣ（Ｃ）ＰＲ降低８０％．ＭＡＰＫ蛋白量变化不受ＡＶＰ（４－８）刺激的影响．在海马切片实验中，也得到一致的结果．结论：ＡＶＰ（４－８）引起的４４ｋＤａＭＡＰＫ活性增高是一种由受体介导的细胞内短时期的酶激活过程，与蛋白质表达量的变化无关．ＭＡＰＫ参与了ＡＶＰ（４－８）诱导的皮层和海马内信号转导途径．
AIM: To investigate the effect of AVP (4-8) (AVP (4-8)) on the changes of mitogen-activated protein kinase (MAPK) activity in rat brain. Methods: After subcutaneous injection of AVP (4-8) into rats, the phosphorylation of MAPK, a specific substrate of MBP, in brain homogenates was determined by column separation. Results: The 44kDa MAPK activity in hippocampus and cerebral cortex of AVP (4-8) -stimulated rats was significantly increased. This increase in activity is reduced by 80% by ZDC (C) PR, an antagonist of AVP (4-8). Changes in MAPK protein levels were unaffected by AVP (4-8) stimulation. In the hippocampal slice experiment, also get the same result. CONCLUSION: Increased 44kDa MAPK activity induced by AVP (4-8) is a receptor-mediated short-term intracellular enzyme activation independent of protein expression. MAPK is involved in AVP (4-8) -induced cortical and hippocampal signal transduction pathways.