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类胰岛素样生长因子结合蛋白(IGFBP)在调节IGF的生物学功能上起着重要的作用,利用简并引物扩增及RACE克隆技术,首次克隆得到大菱鲆IGFBP-1,-2基因cDNA全长序列。两个基因氨基酸序列都分为保守的N端和C端以及高度特异的L区域3个部分,其中N端和C端共包含18个半胱氨酸残基,N端存在一段保守的(GCGCCXXC)结构,C端存在一段保守的(CWCV)结构,这些结构在与IGF相互作用以及调节与IGF结合的亲和性和稳定性起重要作用。组织表达分析表明,igfbp-1主要在肝脏及脾中大量表达,在其他组织中表达量较少,igfbp-2除在肌肉中没有检测到表达外,在其他组织中均有表达,其中肝脏脑、心、肾、肠中表达量较高。广泛的组织表达模式表明IGFBP是通过自分泌和旁分泌来调节IGF的功能。对于胚胎发育期表达分析表明,ig-fbp-1在整个发育期均检测到表达,igfbp-2在胚体期开始有表达,结果表明igfbp-1和igfbp-2在胚胎发育过程中的细胞生长以及组织器官分化都起着重要的生理学作用。
Insulin-like growth factor binding protein (IGFBP) plays an important role in regulating the biological function of IGF. Using degenerate primer amplification and RACE cloning technology, IGFBP-1 and -2 gene cDNAs of turbot were screened for the first time Long sequence. The amino acid sequences of the two genes are divided into three parts: N-terminal and C-terminal, and highly specific L region, which contains 18 cysteine residues at the N-terminus and C-terminus and a conserved region at the N-terminus (GCGCCXXC ) Structure, there is a conserved (CWCV) structure at the C-terminus that plays an important role in interacting with IGFs and in modulating the affinity and stability of binding to IGFs. Tissue expression analysis showed that igfbp-1 was mainly expressed in the liver and spleen, but less in other tissues. Igfbp-2 was expressed in other tissues except for the expression in muscle, except in liver , Heart, kidney, high expression in the intestine. A wide range of tissue expression patterns indicate that IGFBP modulates IGF function through autocrine and paracrine processes. Analysis of embryonic development showed that ig-fbp-1 was detected throughout the developmental stage and igfbp-2 was expressed at the beginning of the embryo stage. The results indicate that igfbp-1 and igfbp-2 are involved in cell growth during embryonic development As well as the differentiation of tissues and organs play an important physiological role.