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合成了２，３－二甲基－１，４，８，９－四氮三联苯（ｄｍ ｔａｔｐ）和２，３－二苯基－１，４，８，９－四氮三联苯（ｄｐｔａｔｐ）两种新配体及它们与 ２，２′－联吡啶和钌（Ⅱ）的混配物［Ｒｕ（ｂｐｙ）２ｄｍ ｔａｔｐ］２＋ （１）和［Ｒｕ（ｂｐｙ）２ｄｐｔａｔｐ］２＋ （２）， 用电子吸收光谱、稳态荧光、粘度测定和圆二色谱研究了配合物与小牛胸腺ＤＮＡ的相互作用． 结合我们以前对配合物［Ｒｕ（ｂｐｙ）２ｔａｔｐ］２＋ （３） （ｔａｔｐ 为１，４，８，９－四氮三联苯）与小牛胸腺ＤＮＡ的作用研究， 得出配合物与ＤＮＡ的键合强度顺序为： ［Ｒｕ（ｂｐｙ）２ｔａｔｐ］２＋ ＞［Ｒｕ（ｂｐｙ）２ｄｐｔａｔｐ］２＋ ＞ ［Ｒｕ（ｂｐｙ）２ｄｍ ｔａｔｐ］２＋ ， 这与插入配体的位阻效应的减少趋势相一致． 同时，还测定了配合物与ＤＮＡ的键合常数．
Synthesis of 2,3-dimethyl-1,4,8,9-tetraaz terphenyl (dm tatp) and 2,3-diphenyl-1,4,8,9-tetraaz terphenyl (dptatp) Two novel ligands and their combination [Ru (bpy) 2dm tatp] 2+ (1) and [Ru (bpy) 2dptatp] 2+ (2) with 2,2’- bipyridine and ruthenium (II) Electron absorption spectroscopy, steady state fluorescence, viscosity measurement and circular dichroism were used to study the interaction between the complex and calf thymus DNA. Combined with our previous studies on the interaction of [Ru (bpy) 2tatp] 2+ (3) (tatp is 1,4,8,9-tetraaza-terphenyl) with calf thymus DNA, The order of the binding intensities is: [Ru (bpy) 2tatp] 2+> [Ru (bpy) 2dptatp] 2+> [Ru (bpy) 2dm tatp] 2+, consistent with the decreasing tendency of the steric effect of the inserted ligands. At the same time, the bonding constant between the complex and DNA was also determined.