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目的用MRI及PET结合观察移植的胚胎干细胞在正常心肌及梗死心肌内的存活、增殖情况,宿主巨噬细胞对抗移植的干细胞引起的磁共振信号改变情况,移植的干细胞引起的心功能改变情况,以及心功能改善是否与胚胎干细胞心肌分化有关。方法所有用于实验的动物都经过动物管理委员会批准,移植的小鼠胚胎干细胞都用HSV1病毒胸腺嘧啶核苷激酶的突变体(HSV1-sr39tk)转染。同时又用超顺磁性氧化铁(SPIO)标记,上述标记的干细胞被注射到梗死心肌的周边带及无胸腺鼠正常心脏的对应部位,4周后依次行MRI及PET检查。
Objective To observe the survival and proliferation of transplanted embryonic stem cells in normal myocardium and infarcted myocardium by MRI and PET, the change of magnetic resonance signals caused by transplanting stem cells by host macrophages, the cardiac function changes induced by transplanted stem cells, And whether improvements in cardiac function are related to myocardial differentiation of embryonic stem cells. Methods All animal experiments were approved by the Animal Stewardship Council. Transplanted mouse embryonic stem cells were transfected with a mutant of HSV1 virus thymidine kinase (HSV1-sr39tk). At the same time, they were labeled with superparamagnetic iron oxide (SPIO). The labeled stem cells were injected into the peripheral zone of the infarcted myocardium and the normal heart of athymic mice. MRI and PET were performed 4 weeks later.