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目的:柯萨奇病毒-腺病毒受体(coxsackie and adenovirus receptor,CAR)介导腺病毒与靶细胞之间的粘附,是腺病毒转染靶细胞的限速因子。本文分析CAR在瘤旁上皮组织、结肠肿瘤原发灶和淋巴结转移灶中的表达,为设计腺病毒载体基因治疗方案提供依据。方法:采用免疫组织化学方法检测62例结肠癌组织中CAR的表达,分析CAR表达与临床病理特征的关系。结果:与瘤旁上皮组织相比,肿瘤组织普遍存在CAR表达下调,但CAR在原发灶和淋巴结转移灶的表达水平无明显差异。CAR的表达水平与患者年龄及肿瘤大小相关。结论:结肠癌组织中存在CAR表达下调,提示在设计腺病毒载体基因治疗方案时应充分考虑CAR表达变化对疗效的影响。
OBJECTIVE: The coxsackie and adenovirus receptor (CAR) mediates the adhesion between adenovirus and target cells and is the rate-limiting factor of adenovirus transfected target cells. This article analyzes the expression of CAR in tumor-adjacent epithelium, primary tumor of colon tumor and lymph node metastasis, and provides the basis for designing adenovirus vector gene therapy program. Methods: Immunohistochemistry was used to detect the expression of CAR in 62 cases of colon cancer. The relationship between CAR expression and clinicopathological features was analyzed. Results: Compared with the tumor-adjacent epithelium, CAR expression was generally down-regulated in the tumor tissue, but there was no significant difference in the expression of CAR between the primary tumor and the lymph node metastasis. The level of CAR expression correlated with patient’s age and tumor size. Conclusion: The down-regulation of CAR expression in colon cancer tissues suggests that the effects of CAR expression changes on the therapeutic effect should be fully considered when designing adenovirus vector gene therapy regimens.