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目的:观察胍丁胺抑制纳洛酮引起小鼠吗啡戒断跳跃与其抑制一氧化氮合酶(NOS)的关系,方法:用测定[~3H]胍氨酸浓度的方法确定NOS活性,结果:在体外胍丁胺底物竞争性抑制正常和吗啡依赖小鼠小脑、端脑和丘脑NOS活性,纳洛酮引起吗啡依赖小鼠戒断跳跃和小脑、端脑、丘脑NOS活性升高,用吗啡和胍丁胺共同处理小鼠显著抑制纳洛酮促使小鼠戒断跳跃和NOS活性升高的作用,咪唑克生抑制胍丁胺的此作用,结论:胍丁胺对纳洛酮引起戒断跳跃的抑制作用与其通过激活咪唑啉受体和底物竞争性抑制NOS活性相关。
AIM: To observe the effect of agmatine on the inhibition of naloxone - induced morphine withdrawal and its inhibition of nitric oxide synthase (NOS) in mice. Methods: The activity of NOS was determined by measuring the concentration of [~ 3H] In vitro, agmatine substrate competitively inhibits NOS activity in cerebellum, telencephalon, and thalamus in normal and morphine-dependent mice. Naloxone causes withdrawal and withdrawal of morphine-dependent mice and increases in NOS activity in the cerebellum, telencephalon, and thalamus, with morphine And agmatine co-treatment of mice significantly inhibited naloxone to promote withdrawal and jump in mice and the role of elevated NOS activity, midazolam inhibition of this effect of agmatine, Conclusion: agmatine on withdrawal caused by naloxone The inhibition of jumping is associated with its ability to competitively inhibit NOS activity by activating imidazolinium receptors and substrates.