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目的探讨环氧合酶抑制剂NS398对子宫内膜癌细胞株的生物学行为的影响。方法2005年9月至2006年11月于华中科技大学同济医学院附属同济医院妇产科实验室用MTT、DNA梯度降解试验及流式细胞仪等方法,研究NS398体外对子宫内膜癌细胞系Ishikawa的抑制增殖、促进凋亡作用。结果NS398对Ishikawa细胞产生明显的生长抑制作用,且表现为剂量依赖性(F=36.598,P<0.01);DNA梯度降解试验、流式细胞仪分析NS398浓度依赖性地诱导了Ishikawa细胞凋亡(F=11.342,P<0.01),且凋亡与细胞周期受阻有关(F=22.445,P<0.01)、主要阻止在G1/S期即DNA合成期。结论NS398对Ishikawa细胞具有显著浓度依赖性的体外生长抑制、诱导凋亡、阻滞DNA合成作用。
Objective To investigate the effect of cyclooxygenase inhibitor NS398 on the biological behavior of endometrial carcinoma cell lines. Methods From September 2005 to November 2006, MTT, DNA gradient degradation assay and flow cytometry were used to study the effect of NS398 on endometrial carcinoma cell lines in vitro and in vivo in the gynecological and obstetrics laboratory of Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology. Ishikawa inhibits proliferation and promotes apoptosis. Results NS398 had a significant inhibitory effect on Ishikawa cells in a dose-dependent manner (F = 36.598, P <0.01). DNA degradation assay and flow cytometry analysis showed that NS398 induced apoptosis in Ishikawa cells in a concentration-dependent manner F = 11.342, P <0.01), and apoptosis was related to cell cycle arrest (F = 22.445, P <0.01). Conclusion NS398 inhibits Ishikawa cells in a concentration-dependent manner in vitro, induces apoptosis and blocks DNA synthesis.