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玉米(Zea mays)杂交种隆平206是黄淮海地区推广的重要玉米品种,具有显著生长优势以及产量优势,为研究杂种优势提供了极好的材料。为了从分子生物学的角度解析隆平206相对于父母本(父本:L7221;母本:L239)具有良好杂种优势的原因,本研究利用RNA-Seq技术,从转录组水平对其进行相关分析。结果表明,在杂交种与父母本之间共检测出3 005个差异表达基因(differentially expressed genes,DEGs),其中1 788个基因在杂交子代中上调表达,925个下调表达。通过对这些差异基因进行基因本体(Gene Ontology,GO)分析和通路富集分析,发现5.88%差异表达的基因参与到碳代谢途径,8.24%的DEGs参与到植物信号转导途径,并且这些DEGs在杂交子代中的表达量显著升高。结果显示,碳代谢途径以及植物信号转导途径在隆平206杂种优势的形成过程中具有重要的意义。研究结果可以为后续研究玉米杂种优势的分子机制提供重要的数据支持。
The hybrid variety Zea mays, Longping 206, is an important maize variety promoted in Huang-Huai-Hai region. It has significant growth and yield advantages and provides an excellent material for studying heterosis. In order to analyze the reason that Longping 206 has good heterosis with parents (father: L7221; female: L239) from the point of view of molecular biology, this study used RNA-Seq technique to analyze the correlation between the level of transcriptome. The results showed that there were 3 005 differentially expressed genes (DEGs) between hybrids and their parents, of which 1 788 genes were up-regulated in hybrid progenies and 925 were down-regulated. Through the Gene Ontology (GO) analysis and pathway enrichment analysis of these differentially expressed genes, it was found that 5.88% differentially expressed genes involved in the carbon metabolic pathway, 8.24% DEGs involved in the plant signal transduction pathway, and these DEGs in the The expression level in hybrid progeny was significantly increased. The results showed that carbon metabolism pathway and plant signal transduction pathway are of great significance in the formation of the heterosis of Longping 206. The results can provide important data support for the further study of the molecular mechanism of maize heterosis.