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将肌红蛋白(Mb)包埋在十六烷基吡啶六氟磷酸盐([CePy][PF6])与透明质酸(HA)混合得到的复合膜内,采用滴涂法将其修饰在玻碳电极(GCE)表面,制备了HA-[CePy][PF6]-Mb/GCE修饰电极,研究了Mb的直接电化学及电催化行为,建立了H2O2的计时安培测定新方法。结果表明,在0.1 mol/LPBS(pH 7.0)中,该修饰电极上产生了一对准可逆的氧化还原峰,电子转移速率常数(ks)为3.9/s,电极表面表观覆盖度(Γ*)为4.36×10-9mol/cm2,表观米氏常数(Km)为2.6×10-5mol/L;该修饰电极上的Mb对H2O2的还原表现出良好的电催化作用,催化电流与H2O2浓度在2.5×10-6～5.0×10-5mol/L范围内呈线性关系,检出限为8.0×10-7mol/L(S/N=3)。
Myoglobin (Mb) was embedded in a composite membrane obtained by mixing cetylpyridinium hexafluorophosphate ([CePy] [PF6]) and hyaluronic acid (HA), which was modified in glass (CeCE) [PF6] -Mb / GCE modified electrode was prepared on the surface of carbon electrode (GCE). The direct electrochemical and electrocatalytic behaviors of Mb were studied. A new method for the determination of H2O2 was developed. The results showed that a pair of quasi-reversible redox peaks were obtained on the modified electrode at 0.1 mol / L (pH 7.0). The electron transfer rate constant (ks) was 3.9 / s, and the apparent surface coverage (Γ * ) Was 4.36 × 10-9mol / cm2, and the apparent Michaelis constant was 2.6 × 10-5mol / L. The Mb on the modified electrode showed good electrocatalytic activity for the reduction of H2O2. The catalytic current and H2O2 concentration The linear range was 2.5 × 10-6 ~ 5.0 × 10-5mol / L with the detection limit of 8.0 × 10-7mol / L (S / N = 3).