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采用SDS/氯仿/苯酚法和oligo(dT)纤维素亲和层析从人胎肝提取poly(A)~+mRNA,注入非洲爪蟾卵母细胞,翻译出肝细胞生长因子(HGF),产物能从卵母细胞中分泌。在9种细胞(包括人与小鼠的4种不同组织和5种细胞系)检测系统中,证明翻译的HGF与直接提取的HGF活性一致,应用滤膜超滤法估计分子量均在10～30kDa,可以初步认为两者是同一物质。从而,支持了人胎肝HGF是胎儿肝脏细胞基因表达的产物。
Poly (A) ~ + mRNA was extracted from human fetal liver by SDS / chloroform / phenol method and oligo (dT) cellulose affinity chromatography, and injected into Xenopus laevis oocytes to transfect hepatocyte growth factor (HGF) Can be secreted from oocytes. In the detection system of 9 kinds of cells (including 4 different tissues and 5 kinds of cell lines of human and mouse), it was proved that the translated HGF is consistent with the activity of directly extracted HGF, and the molecular weight of the translated HGF is estimated to be 10-30 kDa , You can initially think that the two are the same material. Thus, human fetal liver HGF is supported as a product of fetal liver cell gene expression.